High-Performance real-time PCR with LightCycler? Systems

Get precise, consistent results, for any throughput or budget, with our range of instruments, assays, reagents and disposables

High performance real-time PCR:

More than a decade of continuous qPCR innovation with the LightCycler? Systems.

Roche continues to innovate, and with that comes a dedication to the scientists who use the LightCycler? system for their experimental needs. Benefit from Roche's experience in real-time PCR and achieve precise, consistent results with a full range of instruments, reagents, assays, and disposables to fit any laboratory throughput or budget. 

Choose the LightCycler? System that's right for you

 

 

葡京线上游戏app 96 Instrument

See qPCR in a whole new way

Generate data you can trust.

Ideal for all levels of experience, the LightCycler? 96 Instrument provides an ideal combination of temperature homogeneity and data reproducibility. Comprehensive programming options, a large touchscreen, and intuitive analysis software that is accessible to any user make it easy to obtain publication-ready results.
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Discover which real-time PCR System is right for you.

The tools for your research start here.

? LightCycler??2.0* System LightCycler? 96
System
LightCycler? 480 System LightCycler? 1536 System
Reactions per Run 32 96 96/384 1536
PCR Reactions Volume 20??l 10 - 50??l 5-20??l?? 10 - 100??l 0.5 - 2 ?l
Run Time <30 min <40 min <40 min (384) ??<60 min (96) <50 min
Disposables Glass capillaries 96-multiwell plates
8-tube strips
96/384-multiwell plates
8-tube strips
1536-multiwell
plates
Multiplexing Capability 5x 4x 6x 2x
Automation-Capable No No Yes Yes
Light Source Blue LED ?High-Intensity LED ?High-Intensity white LED Xenon Lamp
? ? ? ? ?

* LightCycler? 2.0 Instrument conforms to the European directive for in vitro diagnostic medical devices 98/79/EC. For in vitro diagnostic use.

Order qPCR instruments

Order qPCR instruments

Request a quote for a real-time PCR system.
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Order qPCR reagents

Order qPCR reagents

Optimized reagents for each application.
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Order qPCR assays

Order qPCR assays

Easily design your qPCR assays or function-tested custom qPCR panels.

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Innovative qPCR 葡京正网.

Generate results you can trust.

Amplify your success in real-time PCR applications with the LightCycler? 葡京正网. Choose the system that best suits your needs, with flexible sample volumes and throughputs ranging from 32 to 1,536 samples per run. All of our instruments provide outstanding temperature uniformity and homogeneity, as well as unbiased data capture, enabling you to quickly generate results that are accurate and reproducible.

葡京线上游戏app 96 System

LightCycler? 96 System

See qPCR in a whole new way.
96-well throughput

Ideal for all levels of experience, the LightCycler? 96 Instrument provides an optimal combination of temperature homogeneity and data reproducibility. Comprehensive programming options, a large touchscreen, and intuitive analysis software make it easy to obtain publication-ready results.

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葡京线上游戏app 480 System

LightCycler? 480 System

Versatile for experienced users.
96- to 384-well throughput

The LightCycler? 480 System is a high-performance, flexible throughput qPCR platform (interchangeable 96- or 384-well blocks) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and pathogen detection.

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葡京线上游戏app 1536 System

LightCycler? 1536 System

High throughput - redefined.
1536-well throughput

The LightCycler? 1536 Instrument is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. Take advantage of the first plate-based real-time qPCR instrument that provides 1,536 data points in a single PCR run in less than 50 minutes.

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葡京线上游戏app  2.0 System

LightCycler??2.0 System

A proven standard of excellence.
32-well throughput


This LightCycler? Carousel-Based System has repeatedly set the standard for accurate and fast real-time PCR. It was the first system to introduce hybridization probes, true melting curve analysis, automated absolute quantification, relative quantification with efficiency correction, and more.

LightCycler? 2.0 Instrument conforms to the European directive for in vitro diagnostic medical devices 98/79/EC. For in vitro diagnostic use.

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qPCR Reagents

Designed to maximize the performance of your system.

Rely on Roche proprietary PCR enzymes and formulations to consistently deliver exceptional sensitivity and specificity for your real-time PCR assays.
Choose the reagent that's best for your experiments on:

LightCycler? 葡京正网

Non-Roche qPCR instruments

 

葡京线上游戏app Multiplex Master Mix

LightCycler? Multiplex Masters

Made to discover more.

Bring your qPCR to the next level with the new 5x concentrated multiplex master mixes. Detect a greater number of targets per reaction, work more efficiently, and maximize your valuable samples.

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葡京现金下载_LightCycler

LightCycler? 葡京现金下载 Masters

Witness the evolution of RT-qPCR.

Sensitive, specific and one-vial 葡京现金下载 master mixes turn even the smallest clues into measurably better results, ensuring no evidence gets lost in transcription.

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葡京国际真人赌城

葡京国际真人赌城

Your online search assistant

Not sure which kit is best for you? With the new Roche Reagent Selection guide, you can easily find which real-time PCR reagents are right for your applications.

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qPCR Reagents for Roche 葡京正网

? FastStart Essential DNA Probes Master FastStart Essential DNA Green Master LightCycler? 480 Probes Master LightCycler? 480 SYBR? Green I Master LightCycler? 480 RNA Master Hydrolysis Probes LightCycler? 480 HRM Master LightCycler? Multiplex RNA Virus Master LightCycler? Multiplex DNA Master
网上葡京赌城游戏s Gene expression Gene expression Gene expression Gene expression Gene expression High resolution melting Multiplex pathogen detection Multiplex pathogen detection
Starting Material DNA DNA DNA DNA RNA DNA Viral RNA Pathogen DNA
Chemistry Hydrolysis probes SYBR??Green I? ? Hydrolysis probes SYBR? Green I Hydrolysis probes ResoLight Dye Hydrolysis probes Hydrolysis probes
Instrument Capability LightCycler? 96 Instrument LightCycler??96 Instrument LightCycler? 480 Instrument LightCycler? 480 Instrument LightCycler??96 & LightCycler??480 葡京正网 LightCycler? 96 & LightCycler? 480 葡京正网 LightCycler? 96 & LightCycler? 480 葡京正网 LightCycler? 96 & LightCycler? 480 葡京正网

qPCR Reagents for Non-Roche 葡京正网

Roche Master Mix FastStart Universal Probe Master Mix (ROX) FastStart Universal SYBR? Green Master Mix (ROX) FastStart TaqMan Probe Master FastStart SYBR? Green Master EagleTaq Universal Master Mix (ROX)
网上葡京赌城游戏s Gene expression Gene expression Gene expression Gene expression
Gene expression, 网上澳门葡京官网
Starting Material DNA DNA DNA
DNA DNA
Chemistry Hydrolysis probes SYBR? Green I? ? Hydrolysis probes SYBR? Green I Hydrolysis probes
ROX Yes Yes No No Yes
Instrument Capability non-Roche instruments requiring ROX such as ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT. non-Roche instruments requiring ROX such as ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT.
non-Roche instruments not requiring ROX such as Bio-Rad CFX 96 Touch, RotorGene Q. non-Roche instruments not requiring ROX such as Bio-Rad CFX 96 Touch, RotorGene Q. non-Roche instruments requiring ROX such as ViiA7, StepOne Plus, ABI 7500, ABI 7900 HT.
葡京线上游戏app 1536 System Reagents

LightCycler? 1536 System Reagents

Downscale reagent volumes and accelerate the workflow.

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葡京线上游戏app Carousel System Reagents

LightCycler? Carousel System Reagents

Optimal reagents for rapid reactions in capillaries.
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澳门葡京直营 Kits

澳门葡京直营 Kits

For robust and unbiased cDNA synthesis.
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Genomics Workflow Publications

The 葡京网上真人网址 is your source for relevant, reliable information specific to your research applications.

Explore Database
线上葡京电玩

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Easily design your qPCR assay to analyze over 5 million transcripts of any sequenced organism.


Configure Assay
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葡京线上电子

Function-tested qPCR single assays or custom panels, for human, mouse or rat targets of your choice.
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Configure Panel

qPCR 澳门葡京备用网址 and Panels

Easy to design. Ready to run.

Choose from a variety of Roche probe-based qPCR assay solutions for your gene expression needs. Opt for universal probes for affordable and easy-to-design assays. Or select function-tested single assays and customized panels to skip design and optimization steps. Rely on Roche qPCR assay solutions to generate reliable and consistent real-time PCR results, along with complete assay information to facilitate compliance with the MIQE guidelines.

Choose the assay solution that's best for you

Lightmix for qPCR

LightMix?Modular Pathogen Detection Assay*

Ready to use assays designed for multiplexing.

Choose from an extensive range of assays for pathogen detection. Compatibility with Roche reagents and instruments enables seamless assay integration into your workflow.

*Manufactured by TIB MOLBIOL GmBH, exclusively distributed by Roche

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Create Your Panel

Universal Probe Library for qPCR

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Cost-effective, probe-based assays.

Experience how fast and easy it is to design a specific, cost-effective, hydrolysis probe-based qPCR assay in 3 easy steps using our free online software.

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Get Started

葡京线上电子 澳门葡京备用网址

葡京线上电子 澳门葡京备用网址

Function-tested probe-based assays.

Skip time-consuming assay design and optimization steps with the function-tested 葡京线上电子 qPCR assays for gene expression for human, mouse, rat or other species. Receive ready-to-use probe-based assays, compatible with any real-time PCR instrument

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Design Assay

葡京线上电子 Panel

葡京线上电子 葡京真人电子

Function-tested qPCR assay Panels.

Create your own gene expression panels with your targets of choice on?LightCycler? Plates (96- or 384-well). All assays are function-tested and pre-plated for the upmost convenience and quick time to results.

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Configure Panel

Probe-based qPCR assay solutions available from Roche


Assay
LightMix
?Modular 澳门葡京备用网址
Universal
ProbeLibrary
Single Probes
Universal
ProbeLibrary Library
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Single 澳门葡京备用网址
葡京线上电子
Focus Panels
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Format Predesigned assays for detection of infectious
disease pathogens
(2 vials)
Single probe
(2 vials)
90 most common
probes for human
targets (90 vials)
Custom Assay,
single tube
Pre-configured,
pre-plated
(96- or 384-well)
Custom, pre-plated
(96- or 384-well)
Included
Components
Probe, Primers and
Positive Control
Probe 90 probes Probe and Primers Probes, Primers
and Plates
Probes, Primers
and Plates
Validation Function-tested In silico In silico Function-tested Function-tested Function-tested
Assay
Information
Protocol available
from Roche
Complete assay information provided with all Roche qPCR assays
Instrument Compatibility LightCycler? 480 and?
cobas z 480
qPCR 葡京正网
?detecting FAM
qPCR 葡京正网
detecting FAM
qPCR 葡京正网
detecting FAM
LightCycler? 480
LightCycler? 96
LightCycler??480
LightCycler??96
葡京线上电子 Pre-Amp cDNA Master and Pre-Amp Pirmer pools

Working with limited?amounts of RNA?

Learn more about 葡京线上电子 Pre-Amp cDNA Master and Pre-Amp pirmer pools.

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Reference Gene 澳门葡京备用网址

Reference Gene 澳门葡京备用网址

Easily multiplex with your target UPL assay.? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?

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Reference Gene Panel

Reference Gene?Panel

Identify the reference genes with the least variability for your test system.

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LightCycler? Consumables

Designed to maximize performance.

葡京线上游戏app Multiwell Plates

LightCycler? Multiwell Plates

Optimal fit and performance.

Fully exploit the advanced thermal and optical characteristics of your LightCycler? 96 or 480 Systems with tailor-made LightCycler? Multiwell Plates to allow optimal heat transfer and optical compatibility.

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葡京线上游戏app 8-Tube Strips

LightCycler? 8-Tube Strips

Ideal consumable for low throughput.

Use the LightCycler? 8-Tube Strips as plastics disposables that match exactly your LightCycler? 96 or 480 Systems block geometry for optimal fit and real-time PCR results.

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葡京线上游戏app Capillaries

LightCycler? Capillaries

Optimized for Carousel Systems.

Maximize qPCR performance with optimal heat transfer by relying on the renowned LightCycler? Capillaries for all LightCycler? Carousel Systems.

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葡京线上游戏app Multiwell Plates

Want to simplify plate tracking?

Rely on LightCycler??Multiwell Plates with 4 barcodes for tracking and automation.

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葡京线上游戏app 1536 Multiwell Plates

LightCycler??1536 Multiwell Plate

Increase your throughput with 1,536 data points per run.

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To download the LightCycler? Software, please first register by completing this form. You will receive an email with a link for downloading the software installer, demo data files and the user guide pdf.

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For LightCycler? 480 System Customers

Please contact your Roche representative for the following upgrade scenario:

1) Upgrading from Windows XP to Windows 7 using Data Station HP rp5700

2) Upgrading from Windows XP to Windows 7 using Data Station other than HP rp5700

3) Using Software Version other than 1.5 and upgrading to 1.5.1.

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For LightCycler? Carousel-Based System Customers

Please note that the LightCycler? QC Test Software 3.0 is for life science research only and not for use in diagnostics procedures. In case you need to qualify IVD LightCycler? Instrument, contact your Roche representative.

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